🧬 Heredity | Mendel’s Monohybrid Cross
Class 10 Science | Chapter – Heredity | CBSE + Foundation + Competitive Level
🧬 Mendel’s Monohybrid Cross
1. What is a Monohybrid Cross?
A monohybrid cross is a genetic cross in which the inheritance of one pair of contrasting traits is studied at a time.
For example, Mendel studied the inheritance of:
For example, Mendel studied the inheritance of:
- Tallness of pea plant
- Dwarfness of pea plant
Mono = One
Hybrid = Offspring produced from genetically different parents.
Therefore, a monohybrid cross studies inheritance of one character/pair of contrasting traits.
Hybrid = Offspring produced from genetically different parents.
Therefore, a monohybrid cross studies inheritance of one character/pair of contrasting traits.
2. Gregor Johann Mendel
Gregor Johann Mendel was an Austrian monk and scientist who performed experiments on pea plants.
He is widely known as the:
He is widely known as the:
Father of Genetics
Mendel selected the garden pea plant:
Pisum sativum
for his experiments.
3. Why Did Mendel Choose Pea Plants?
Mendel selected pea plants because:
- They have many easily observable contrasting traits.
- They have a relatively short generation time.
- They can self-pollinate.
- Artificial cross-pollination can be performed easily.
- Large numbers of offspring can be obtained.
- Pure-breeding varieties were available.
4. Contrasting Traits in Pea Plants
| Character | Contrasting Forms |
|---|---|
| Plant height | Tall / Dwarf |
| Seed shape | Round / Wrinkled |
| Seed colour | Yellow / Green |
| Flower colour | Violet / White |
| Pod shape | Inflated / Constricted |
| Pod colour | Green / Yellow |
| Flower position | Axial / Terminal |
| Stem length | Long / Short |
5. Symbols Used in Monohybrid Cross
Let:
Therefore: TT = Homozygous tall
Tt = Heterozygous tall
tt = Homozygous dwarf
T
= Dominant allele for tallness
t
= Recessive allele for dwarfness
Therefore: TT = Homozygous tall
Tt = Heterozygous tall
tt = Homozygous dwarf
6. Parental Generation – P Generation
Mendel crossed a pure tall pea plant with a pure dwarf pea plant.
Pure tall plant: TT
Pure dwarf plant: tt
Pure tall plant: TT
Pure dwarf plant: tt
P Generation:
TT × tt
TT × tt
7. Formation of Gametes
The alleles separate during gamete formation.
A TT plant can produce only T gametes.
A tt plant can produce only t gametes.
TT
→
T
T
tt
→
t
t
8. F₁ Generation
When the gametes fuse:
T × t → Tt
All F₁ offspring have genotype:
Tt
Since T is dominant, all F₁ plants are:
Tall
TT × tt
↓
All F₁ = Tt
Phenotype = All Tall
↓
All F₁ = Tt
Phenotype = All Tall
9. Mendel’s F₁ Observation
Mendel observed that only one of the two contrasting traits appeared in the F₁ generation.
The tall characteristic appeared, while dwarfness was not visible.
This led to the concept of: Dominant and Recessive traits.
The tall characteristic appeared, while dwarfness was not visible.
This led to the concept of: Dominant and Recessive traits.
The recessive trait did not disappear genetically. Its allele remained present in the F₁ plants as part of the heterozygous genotype Tt.
10. Self-Pollination of F₁ Plants
Mendel allowed F₁ plants to self-pollinate.
Each F₁ plant had genotype:
Tt
Therefore:
Tt × Tt
Each parent can produce two types of gametes:
T and t
11. Punnett Square – Tt × Tt
| Gametes | T | t |
|---|---|---|
| T |
TT
Tall |
Tt
Tall |
| t |
Tt
Tall |
tt
Dwarf |
12. Genotypic Ratio
The four possible genotypes are:
- TT
- Tt
- Tt
- tt
Genotypic Ratio = 1 TT : 2 Tt : 1 tt
13. Phenotypic Ratio
TT = Tall
Tt = Tall
Tt = Tall
tt = Dwarf
Therefore:
Tt = Tall
Tt = Tall
tt = Dwarf
Therefore:
Phenotypic Ratio = 3 Tall : 1 Dwarf
14. Complete Monohybrid Cross
P
→
TT × tt
↓
F₁
→
All Tt
→
Tall
↓ Selfing
Tt × Tt
↓
TT
Tt
Tt
tt
3 Tall : 1 Dwarf
15. Law of Segregation
Mendel proposed that the two factors/alleles for a character separate during gamete formation.
Thus a heterozygous plant: Tt produces: T and t gametes.
This principle is called the: Law of Segregation
Thus a heterozygous plant: Tt produces: T and t gametes.
This principle is called the: Law of Segregation
Tt → T + t
16. Law of Dominance – Basic Idea
When two contrasting alleles occur together in a heterozygote, one may express itself while the other remains masked.
Example: Tt → Tall Here T is dominant and t is recessive.
Example: Tt → Tall Here T is dominant and t is recessive.
17. Pure-Breeding Plants
A pure-breeding organism produces offspring with the same form of a trait when self-pollinated under the relevant conditions.
For the simple pea-height example:
TT → pure tall
tt → pure dwarf
tt → pure dwarf
18. Importance of Monohybrid Cross
A monohybrid cross helps us understand:
- Inheritance of a single characteristic.
- Dominant and recessive traits.
- Genotype and phenotype.
- Homozygous and heterozygous conditions.
- Segregation of alleles.
- Probability of different offspring genotypes.
- Why recessive traits can reappear in later generations.
19. Probability in Monohybrid Cross
For:
Tt × Tt
The four equally likely allele combinations in the simple Mendelian model are:
Probability of Tt = 2/4 = 50%
Probability of tt = 1/4 = 25%
Thus the probability of tall phenotype is: 3/4 = 75% and dwarf phenotype: 1/4 = 25%
TT, Tt, Tt, tt
Therefore:
Probability of TT = 1/4 = 25%
Probability of Tt = 2/4 = 50%
Probability of tt = 1/4 = 25%
Thus the probability of tall phenotype is: 3/4 = 75% and dwarf phenotype: 1/4 = 25%
20. Complete Result Table
| Offspring | Genotype | Phenotype | Probability |
|---|---|---|---|
| 1 | TT | Tall | 25% |
| 2 | Tt | Tall | 25% |
| 3 | Tt | Tall | 25% |
| 4 | tt | Dwarf | 25% |
21. Genotypic Ratio vs Phenotypic Ratio
| Ratio | Meaning | Monohybrid F₂ |
|---|---|---|
| Genotypic Ratio | Ratio of genetic combinations | 1 : 2 : 1 |
| Phenotypic Ratio | Ratio of observable traits | 3 : 1 |
22. 30 MCQs – Mendel’s Monohybrid Cross
Q1. A monohybrid cross studies:
A) One pair of contrasting traits
B) Two pairs of contrasting traits
C) Three traits
D) All traits simultaneously
Answer: A
Q2. Mendel performed his famous experiments mainly on:
A) Rose
B) Garden pea
C) Wheat
D) Maize
Answer: B
Q3. The scientific name of garden pea is:
A) Pisum sativum
B) Oryza sativa
C) Zea mays
D) Triticum aestivum
Answer: A
Q4. If T is dominant for tallness, the genotype of a pure tall plant is:
A) Tt
B) tt
C) TT
D) T
Answer: C
Q5. The genotype of a pure dwarf plant is:
A) TT
B) Tt
C) tt
D) T
Answer: C
Q6. TT × tt produces:
A) All TT
B) All Tt
C) All tt
D) TT and tt only
Answer: B
Q7. The phenotype of Tt is:
A) Dwarf
B) Tall
C) Both tall and dwarf
D) Cannot be determined
Answer: B
Q8. F₁ generation obtained from TT × tt is:
A) All tall
B) All dwarf
C) 3 tall : 1 dwarf
D) 1 tall : 1 dwarf
Answer: A
Q9. The genotype of an F₁ plant in TT × tt is:
A) TT
B) tt
C) Tt
D) TTT
Answer: C
Q10. F₁ self-cross in a monohybrid experiment is:
A) TT × TT
B) tt × tt
C) Tt × Tt
D) TT × tt
Answer: C
Q11. The F₂ genotypic ratio is:
A) 3:1
B) 1:1
C) 1:2:1
D) 9:3:3:1
Answer: C
Q12. The F₂ phenotypic ratio under complete dominance is:
A) 1:2:1
B) 3:1
C) 1:1
D) 2:1
Answer: B
Q13. In Tt × Tt, the probability of tt is:
A) 25%
B) 50%
C) 75%
D) 100%
Answer: A
Q14. In Tt × Tt, the probability of TT is:
A) 50%
B) 25%
C) 75%
D) 100%
Answer: B
Q15. In Tt × Tt, the probability of heterozygous offspring is:
A) 25%
B) 75%
C) 50%
D) 100%
Answer: C
Q16. Which law explains the separation of alleles during gamete formation?
A) Law of dominance
B) Law of segregation
C) Law of independent assortment
D) Law of conservation
Answer: B
Q17. A Tt plant produces which gametes?
A) Only T
B) Only t
C) T and t
D) TT and tt
Answer: C
Q18. A TT plant produces:
A) Only T gametes
B) Only t gametes
C) T and t gametes
D) No gametes
Answer: A
Q19. A tt plant produces:
A) Only T
B) Only t
C) T and t
D) TT
Answer: B
Q20. Which generation is obtained immediately after crossing P generation?
A) F₂
B) F₁
C) F₃
D) F₄
Answer: B
Q21. In the cross TT × tt, the phenotype of F₁ is:
A) All dwarf
B) All tall
C) 50% tall
D) 25% tall
Answer: B
Q22. Which genotype represents heterozygous condition?
A) TT
B) tt
C) Tt
D) T
Answer: C
Q23. Which genotype represents homozygous recessive?
A) TT
B) Tt
C) tt
D) T
Answer: C
Q24. The 3:1 ratio is observed in:
A) F₁ genotype
B) F₂ phenotype
C) P genotype
D) Gametes
Answer: B
Q25. The 1:2:1 ratio represents:
A) F₂ genotypes
B) F₁ phenotypes
C) P phenotypes
D) Gamete ratio of TT
Answer: A
Q26. Which is the correct sequence?
A) P → F₂ → F₁
B) F₁ → P → F₂
C) P → F₁ → F₂
D) F₂ → F₁ → P
Answer: C
Q27. Why does dwarfness reappear in F₂?
A) Dwarf allele is newly created
B) Recessive alleles combine as tt
C) Tall allele disappears
D) Mutation is necessary
Answer: B
Q28. Mendel is known as:
A) Father of Evolution
B) Father of Genetics
C) Father of Ecology
D) Father of Anatomy
Answer: B
Q29. Which ratio is expected when Tt is crossed with tt?
A) 3 Tall : 1 Dwarf
B) All Tall
C) 1 Tall : 1 Dwarf
D) All Dwarf
Answer: C
Q30. The main purpose of a Punnett square is to:
A) Measure plant height
B) Predict possible genetic combinations in offspring
C) Identify cell organelles
D) Measure DNA length
Answer: B
23. 30 Subjective Questions with Answers
2 Marks
Q1. What is a monohybrid cross?
Answer:
A monohybrid cross is a cross in which inheritance of one pair of contrasting traits is studied.
2 Marks
Q2. Who was Gregor Mendel?
Answer:
Gregor Mendel was a scientist who studied inheritance using garden pea plants. He is known as the Father of Genetics.
2 Marks
Q3. What is the genotype of a pure tall pea plant?
Answer:
TT, assuming T represents the dominant allele for tallness.
2 Marks
Q4. What is the genotype of a pure dwarf pea plant?
Answer:
tt, assuming t represents the recessive allele for dwarfness.
2 Marks
Q5. What is the F₁ genotype in TT × tt?
Answer:
All F₁ offspring are Tt.
3 Marks
Q6. Why did Mendel select pea plants?
Answer:
Pea plants have clear contrasting traits, short generation time, easy self-pollination and controlled cross-pollination, and produce many offspring.
3 Marks
Q7. Define P, F₁ and F₂ generations.
Answer:
P generation is the parental generation. F₁ is the first filial generation produced from P. F₂ is the second filial generation generally obtained by selfing/crossing F₁ individuals in Mendel's experiment.
3 Marks
Q8. What happens when TT is crossed with tt?
Answer:
TT produces only T gametes and tt produces only t gametes. Therefore all offspring are Tt and show the dominant tall phenotype.
3 Marks
Q9. What are the gametes produced by Tt?
Answer:
A Tt plant produces two types of gametes: T and t.
3 Marks
Q10. State the genotypic and phenotypic ratios of Tt × Tt.
Answer:
Genotypic ratio = 1 TT : 2 Tt : 1 tt.
Phenotypic ratio = 3 Tall : 1 Dwarf.
4 Marks
Q11. Explain the F₁ generation of Mendel's monohybrid cross.
Answer:
Mendel crossed pure tall TT with pure dwarf tt. TT produces T gametes and tt produces t gametes. All offspring receive T from one parent and t from the other, giving Tt. Since T is dominant, all F₁ plants are tall.
4 Marks
Q12. Explain the F₂ generation of a monohybrid cross.
Answer:
F₁ plants are Tt. On self-pollination, Tt × Tt gives TT, Tt, Tt and tt. Therefore the genotypic ratio is 1:2:1 and the phenotypic ratio is 3 Tall:1 Dwarf.
4 Marks
Q13. What is the significance of the 3:1 ratio?
Answer:
The 3:1 ratio represents the expected phenotypic ratio in the F₂ generation of a simple monohybrid cross showing complete dominance.
4 Marks
Q14. What is the significance of the 1:2:1 ratio?
Answer:
The 1:2:1 ratio is the expected genotypic ratio of the F₂ generation in a simple monohybrid cross.
4 Marks
Q15. Explain the Law of Segregation.
Answer:
The two alleles of a gene separate during gamete formation. Thus a heterozygous Tt organism forms T and t gametes.
5 Marks
Q16. Draw and explain the Punnett square for Tt × Tt.
Answer:
The parents produce T and t gametes.
Genotypic ratio = 1:2:1.
Phenotypic ratio = 3:1.
| × | T | t |
|---|---|---|
| T | TT | Tt |
| t | Tt | tt |
5 Marks
Q17. Explain why all F₁ plants are tall in TT × tt.
Answer:
TT produces only T gametes and tt produces only t gametes. All offspring therefore have Tt genotype. Since T is dominant over t, all F₁ plants are tall.
5 Marks
Q18. Explain why dwarf plants appear in F₂.
Answer:
F₁ plants are Tt. During gamete formation T and t separate. When two t gametes fuse, the offspring becomes tt. This homozygous recessive genotype expresses dwarfness.
5 Marks
Q19. Differentiate between genotypic and phenotypic ratios.
Answer:
Genotypic ratio compares genetic combinations, such as 1 TT : 2 Tt : 1 tt. Phenotypic ratio compares visible characteristics, such as 3 Tall : 1 Dwarf.
5 Marks
Q20. Explain the role of dominance in a monohybrid cross.
Answer:
When T and t occur together in Tt, T is expressed and t is masked. Thus Tt plants are tall. This dominance relationship results in the 3:1 phenotypic ratio in the F₂ generation.
6 Marks
Q21. Describe Mendel's complete monohybrid cross using plant height.
Answer:
Mendel crossed pure tall TT with pure dwarf tt.
P: TT × tt
All F₁: Tt → Tall
The F₁ plants were self-pollinated:
Tt × Tt
F₂:
TT, Tt, Tt, tt
Genotypic ratio:
1 TT : 2 Tt : 1 tt
Phenotypic ratio:
3 Tall : 1 Dwarf.
6 Marks
Q22. Explain Mendel's observation in F₁ and F₂ generations.
Answer:
In F₁, only tall plants appeared because the dominant allele T masked t.
In F₂, dwarf plants reappeared because the recessive alleles separated in F₁ gamete formation and two t alleles could combine.
Thus F₂ showed 3 tall and 1 dwarf phenotype.
6 Marks
Q23. Explain the inheritance of tallness using a Punnett square.
Answer:
For Tt × Tt:
TT and Tt are tall; tt is dwarf.
Therefore phenotypic ratio = 3:1.
| Gametes | T | t |
|---|---|---|
| T | TT | Tt |
| t | Tt | tt |
6 Marks
Q24. Explain why the recessive trait is not visible in F₁ but appears in F₂.
Answer:
The recessive allele is present in F₁ as Tt but is masked by T. During gamete formation the alleles segregate. In F₂, two recessive t alleles may combine to form tt. Hence the recessive phenotype becomes visible.
6 Marks
Q25. Explain the difference between homozygous and heterozygous individuals in a monohybrid cross.
Answer:
TT and tt contain identical alleles and are homozygous. Tt contains different alleles and is heterozygous. TT is tall, tt is dwarf and Tt is tall when T is dominant.
6 Marks
Q26. Explain how the Law of Segregation is demonstrated by a monohybrid cross.
Answer:
F₁ plants are Tt. During gamete formation, T and t separate. Therefore the gametes carry either T or t. Their random combination during fertilisation produces TT, Tt and tt offspring in F₂. This demonstrates segregation of alleles.
6 Marks
Q27. A tall plant is crossed with a dwarf plant and all offspring are tall. What could be the genotype of the tall parent?
Answer:
If all offspring are tall and the dwarf parent is tt, the tall parent could be TT in the standard monohybrid example.
TT × tt → all Tt → all tall.
6 Marks
Q28. A tall plant crossed with a dwarf plant produces both tall and dwarf offspring. Explain.
Answer:
The tall parent must be heterozygous Tt.
Tt × tt
Gametes: T, t and t.
Offspring:
50% Tt → Tall
50% tt → Dwarf
Therefore both phenotypes occur.
6 Marks
Q29. If 400 offspring are produced in Tt × Tt, how many dwarf plants are expected theoretically?
Answer:
Probability of tt = 1/4.
Therefore:
400 × 1/4 = 100
Approximately 100 dwarf plants are expected in the ideal theoretical ratio.
6 Marks
Q30. Explain why actual offspring numbers may not exactly follow a 3:1 ratio.
Answer:
The 3:1 ratio is a theoretical expectation based on probability. Actual offspring numbers can deviate due to chance, sample size and biological factors. With larger sample sizes, observed ratios may tend to approach the expected ratio more closely.
24. HOTS / Competency-Based Questions
HOTS 1.
Two tall plants produce a dwarf offspring. What can you conclude about the parents?
Answer:
Both parents must carry the recessive allele. In the simple model, both are Tt.
HOTS 2.
Why can two tall plants have different genotypes?
Answer:
A tall phenotype can result from TT or Tt when T is dominant.
HOTS 3.
Why is the F₂ phenotypic ratio 3:1 but genotypic ratio 1:2:1?
Answer:
TT and Tt have the same dominant phenotype, so three genotypes correspond to tallness while only tt is dwarf. Hence phenotype 3:1, but genotype remains 1:2:1.
HOTS 4.
If a Tt plant produces 100 gametes theoretically, what proportion would carry T and t?
Answer:
Approximately 50% T and 50% t in the simple Mendelian model.
HOTS 5.
A student says, “The dwarf allele disappears in F₁.” Is this correct?
Answer:
No. The allele remains present in Tt plants but its expression is masked by the dominant allele.
25. Assertion–Reason
Q1.
Assertion: All F₁ plants in TT × tt are tall.
Reason: All F₁ plants have genotype Tt and T is dominant.
Reason: All F₁ plants have genotype Tt and T is dominant.
Answer: Both Assertion and Reason are true, and the Reason correctly explains the Assertion.
Q2.
Assertion: The F₂ phenotypic ratio is 3:1 in a simple monohybrid cross.
Reason: TT and Tt show the same dominant phenotype.
Reason: TT and Tt show the same dominant phenotype.
Answer: Both statements are true, and the Reason correctly explains the Assertion.
Q3.
Assertion: Tt produces only T gametes.
Reason: The two alleles segregate during gamete formation.
Reason: The two alleles segregate during gamete formation.
Answer: Assertion is false, but Reason is true.
Q4.
Assertion: The F₂ genotypic ratio is 1:2:1.
Reason: Tt × Tt can produce TT, Tt, Tt and tt combinations.
Reason: Tt × Tt can produce TT, Tt, Tt and tt combinations.
Answer: Both statements are true, and the Reason correctly explains the Assertion.
Q5.
Assertion: A recessive allele is destroyed in F₁.
Reason: The recessive phenotype is masked in a heterozygous individual.
Reason: The recessive phenotype is masked in a heterozygous individual.
Answer: Assertion is false, but Reason is true.
26. Important Monohybrid Cross Diagram
27. CBSE Golden Points
- Monohybrid cross studies one pair of contrasting traits.
- Mendel used garden pea plants.
- TT = homozygous dominant.
- tt = homozygous recessive.
- Tt = heterozygous.
- TT × tt → all Tt.
- All F₁ plants are tall in this example.
- F₁ self-cross = Tt × Tt.
- F₂ genotypic ratio = 1:2:1.
- F₂ phenotypic ratio = 3:1.
- Tt produces T and t gametes.
- Law of Segregation explains separation of alleles.
- Recessive allele is not destroyed in F₁.
- Dominant phenotype may have TT or Tt genotype.
- Probability of tt in Tt × Tt = 25%.
- Probability of tall phenotype = 75%.
28. Quick Revision Table
| Stage | Cross / Result | Important Point |
|---|---|---|
| P | TT × tt | Pure parents |
| Gametes | T and t | Alleles segregate |
| F₁ | All Tt | All Tall |
| F₁ Selfing | Tt × Tt | Both T and t gametes |
| F₂ Genotype | TT, Tt, Tt, tt | 1:2:1 |
| F₂ Phenotype | 3 Tall : 1 Dwarf | 3:1 |
29. Memory Trick
“TT → Tt → 3:1” Trick
TT × tt ↓ All Tt ↓ Tt × Tt ↓ 1 TT : 2 Tt : 1 tt ↓ 3 Tall : 1 Dwarf
Remember: Genotype = 1:2:1
Phenotype = 3:1
TT × tt ↓ All Tt ↓ Tt × Tt ↓ 1 TT : 2 Tt : 1 tt ↓ 3 Tall : 1 Dwarf
Remember: Genotype = 1:2:1
Phenotype = 3:1
30. One-Line Revision
TT × tt → All Tt → Tt × Tt → 1 TT : 2 Tt : 1 tt → 3 Tall : 1 Dwarf
🧬 Final Summary – Mendel’s Monohybrid Cross
Mendel's monohybrid cross demonstrates the inheritance of one pair of contrasting traits.
P: TT × tt
F₁: All Tt → Tall
F₂: TT, Tt, Tt, tt
Genotypic Ratio = 1 : 2 : 1
Phenotypic Ratio = 3 : 1
Law of Segregation → Alleles separate during gamete formation.
Mendel's monohybrid cross demonstrates the inheritance of one pair of contrasting traits.
P: TT × tt
F₁: All Tt → Tall
F₂: TT, Tt, Tt, tt
Genotypic Ratio = 1 : 2 : 1
Phenotypic Ratio = 3 : 1
Law of Segregation → Alleles separate during gamete formation.